mouse  (Proteintech)

Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

Article Snippet: The anti-mCII ELISA kits were used according to manufacturer's protocol (2036T; Chondrex).

Techniques: Indirect ELISA

Journal: Future Science OA

Article Title: The potential of plant-derived exosome-like nanovesicles from Centella asiatica leaves (CA-PDENs) for anti-inflammation and prevention of UVB-induced photoaging

doi: 10.1080/20565623.2026.2654777

Figure Lengend Snippet: Results of antioxidant and pro-collagen effects of CA-PDENs. (A) Graphical depiction of DPPH radical scavenging activity of CA-PDENs and vitamin C within the concentration of 6.25-400 µg/mL, data are presented as mean ± standard deviation (n = 3). (B) Fluorescence intensity in UVB-induced 1BR3 cells represents the level of intracellular ROS after CA-PDENs treatment for 24 hours; data are presented as mean ± standard deviation (n = 3). *p < 0.05, **p-value < 0.01 vs. negative control. (C) Confocal microscopy images showing collagen type I expression in 1BR3 cells after 6 hours of CA-PDENs incubation at the concentrations of 40 and 100 µg/mL (200× magnification). Nuclei were stained with DAPI (blue), while collagen type 1 was labeled with Alexa Fluor 647 (red). Merged images show the localization of collagen type 1 expression in the cytoplasmic region surrounding the nuclei. (D) Collagen type I levels in UVB-induced 1BR3 cells followed by CA-PDENs incubation at 40 and 100 µg/mL for 48 hours, data are presented as mean ± standard deviation (n = 3). **p-value < 0.01 vs. control. Abbreviations: CA-PDENs, Centella asiatica -derived exosome-like nanovesicles; DPPH, 2,2-diphenyl-1-picrylhydrazyl; ROS, reactive oxygen species; UVB, ultraviolet B; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: Cells were incubated overnight at 4 °C with a mouse anti-collagen type I primary antibody (1:500 in 1% BSA; Invitrogen, USA), followed by incubation with an Alexa Fluor 647-conjugated goat anti-mouse IgG secondary antibody (1:500 in 1% BSA; Abcam, USA) for 60 minutes in the dark.

Techniques: Activity Assay, Concentration Assay, Standard Deviation, Fluorescence, Negative Control, Confocal Microscopy, Expressing, Incubation, Staining, Labeling, Control, Derivative Assay